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Title: High-copy overexpression screening reveals PDR5 as the main doxorubicin resistance gene in yeast
Authors: Demir, Ayşe Banu
Koç, Ahmet
Keywords: Doxorubicin
Genomic DNA
Messenger RNA
PDR5 protein
Saccharomyces cerevisiae
Publisher: Public Library of Science
Source: Demir, A.B., and Koç, A. (2015). High-copy overexpression screening reveals PDR5 as the main doxorubicin resistance gene in yeast. PLoS ONE, 10-12. doi:10.1371/journal.pone.0145108
Abstract: Doxorubicin is one of the most potent anticancer drugs used in the treatment of various cancer types. The efficacy of doxorubicin is influenced by the drug resistance mechanisms and its cytotoxicity. In this study, we performed a high-copy screening analysis to find genes that play a role in doxorubicin resistance and found several genes (CUE5, AKL1, CAN1, YHR177W and PDR5) that provide resistance. Among these genes, overexpression of PDR5 provided a remarkable resistance, and deletion of it significantly rendered the tolerance level for the drug. Q-PCR analyses suggested that transcriptional regulation of these genes was not dependent on doxorubicin treatment. Additionally, we profiled the global expression pattern of cells in response to doxorubicin treatment and highlighted the genes and pathways that are important in doxorubicin tolerance/toxicity. Our results suggest that many efflux pumps and DNA metabolism genes are upregulated by the drug and required for doxorubicin tolerance.
URI: https:/
ISSN: 1932-6203
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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