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Title: Cultivar origin and admixture detection in Turkish olive oils by SNP-based CAPS assays
Authors: Uncu, Ali Tevfik
Frary, Anne
Doğanlar, Sami
Keywords: Food authentication
Molecular markers
Monovarietal olive oil
Olea europaea
Issue Date: 4-Mar-2015
Publisher: American Chemical Society
Source: Uncu, A.T., Frary, A., and Doğanlar, S. (2015). Cultivar origin and admixture detection in Turkish olive oils by SNP-based CAPS assays. Journal of Agricultural and Food Chemistry, 63(8), 2284-2295. doi:10.1021/acs.jafc.5b00090
Abstract: The aim of this study was to establish a DNA-based identification key to ascertain the cultivar origin of Turkish monovarietal olive oils. To reach this aim, we sequenced short fragments from five olive genes for SNP (single nucleotide polymorphism) identification and developed CAPS (cleaved amplified polymorphic DNA) assays for SNPs that alter restriction enzyme recognition motifs. When applied on the oils of 17 olive cultivars, a maximum of five CAPS assays were necessary to discriminate the varietal origin of the samples. We also tested the efficiency and limit of our approach for detecting olive oil admixtures. As a result of the analysis, we were able to detect admixing down to a limit of 20%. The SNP-based CAPS assays developed in this work can be used for testing and verification of the authenticity of Turkish monovarietal olive oils, for olive tree certification, and in germplasm characterization and preservation studies.
ISSN: 0021-8561
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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