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Title: Synthesis of adsorbents with dendronic structures for protein hydrophobic interaction chromatography
Authors: Mata-Gomez, Marco A.
Yaman, Sena
Valencia-Gallegos, Jesus A.
Tarı, Canan
Rito-Palomares, Marco
Gonzalez-Valdez, Jose
Keywords: Adsorbent
Hydrophobic interaction chromatography
Issue Date: 22-Apr-2016
Publisher: Elsevier Ltd.
Source: Mata-Gomez, M. A., Yaman, S., Valencia-Gallegos, J. A.,Tarı, C., Rito-Palomares, M. A., and Gonzalez-Valdez, J. (2016). Synthesis of adsorbents with dendronic structures for protein hydrophobic interaction chromatography. Journal of Chromatography A, 1443, 191-200. doi:10.1016/j.chroma.2016.03.057
Abstract: Here, we introduced a new technology based on the incorporation of dendrons-branched chemical structures-onto supports for synthesis of HIC adsorbents. In doing so we studied the synthesis and performance of these novel HIC dendron-based adsorbents. The adsorbents were synthesized in a facile two-step reaction. First, Sepharose 4FF (R) was chemically modified with polyester dendrons of different branching degrees i.e. third (G3) or fifth (G5) generations. Then, butyl-end valeric acid ligands were coupled to dendrons via ester bond formation. UV-vis spectrophotometry and FTIR analyses of the modified resins confirmed the presence of the dendrons and their ligands on them. Inclusion of dendrons allowed the increment of ligand density, 82.5 ± 11 and 175.6 ± 5.7 μmol ligand/mL resin for RG3 and RG5, respectively. Static adsorption capacity of modified resins was found to be ~60 mg BSA/mL resin. Interestingly, dynamic binding capacity was higher at high flow rates, 62.5 ± 0.8 and 58.0 ± 0.5 mg/mL for RG3 and RG5, respectively. RG3 was able to separate lipase, β-lactoglobulin and α-chymotrypsin selectively as well as fractionating of a whole proteome from yeast. This innovative technology will improve the existing HIC resin synthesis methods. It will also allow the reduction of the amount of adsorbent used in a chromatographic procedure and thus permit the use of smaller columns resulting in faster processes. Furthermore, this method could potentially be considered as a green technology since both, dendrons and ligands, are formed by ester bonds that are more biodegradable allowing the disposal of used resin waste in a more ecofriendly manner when compared to other exiting resins.
ISSN: 0021-9673
Appears in Collections:Food Engineering / Gıda Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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