Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6354
Title: Partial purification of a polygalacturonase from a new Aspergillus sojae mutant and its application in grape mash maceration
Authors: Yıldız, Semanur
Mata-Gomez, Marco A.
Tarı, Canan
Rito-Palomares, Marco
Keywords: Aspergillus sojae
C. Sauvignon
Maceration
Phenolic compounds
Polygalacturonase
Purification
Issue Date: Mar-2017
Publisher: John Wiley and Sons Inc.
Source: Yıldız, S., Mata-Gomez, M. A., Tarı, C., and Rito-Palomares, M. (2017). Partial purification of a polygalacturonase from a new Aspergillus sojae mutant and its application in grape mash maceration. International Journal of Food Science and Technology, 52(3), 834-842. doi:10.1111/ijfs.13346
Abstract: The use of polygalacturonase (PG) preparations in winemaking promotes the release of phenolic compounds. A PG from a new source, Aspergillus sojae mutant, was semi-purified and tested for grape mash maceration. Crude extract (CE), a commercial pectinase, and two high PG activity semi-purified preparations, FI and FII , were applied for maceration at PG activity of 3.5 U g−1 of grape for 46 h. Enzyme-assisted maceration significantly (P < 0.05) increased the total phenolic content from 255.8 to 916.3 ± 5.2, 5732.9 ± 9.9, 563.4 ± 6.7 and 620.6 ± 18.4 mg L−1 for CE, commercial pectinase, FI and FII, respectively. The content of individual phenolics such as gallic, protocatechuic, chlorogenic and p-coumaric acids was improved. Principal component and hierarchical clustering analyses suggested that CE has a better performance upon the release of phenols. Semi-purified preparations acted similar to commercial pectinase. These findings open an opportunity for the potential use of PG from the mutant strain as an alternative macerating enzyme.
URI: http://doi.org/10.1111/ijfs.13346
http://hdl.handle.net/11147/6354
ISSN: 0950-5423
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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