Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6843
Title: Cellular distribution of invadopodia is regulated by nanometer scale surface protein patterns
Authors: Batı Ayaz, Gizem
Can, Ali
Pesen Okvur, Devrim
Batı Ayaz, Gizem
Can, Ali
Pesen Okvur, Devrim
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Electron beam lithography
Nanopattern
Invadopodia
Cortactin
Golgi complex
Issue Date: Oct-2017
Publisher: Elsevier Ltd.
Source: Batı Ayaz, G., Can, A., and Pesen Okvur, D (2017). Cellular distribution of invadopodia is regulated by nanometer scale surface protein patterns. European Journal of Cell Biology, 96(7), 673-684. doi:10.1016/j.ejcb.2017.08.001
Abstract: Invadopodia are proteolytic structures formed by cancer cells. It is not known whether their cellular distribution can be regulated by the organization of the extracellular matrix or the organization of the golgi complex or whether they have an adhesion requirement. Here, we used electron beam lithography to fabricate fibronectin (FN) nanodots with isotropic and gradient micrometer scale spacings on K-casein and laminin backgrounds. Investigating cancer cells cultured on protein nanopatterns, we showed that (i) presence of FN nanodots on a K-casein background decreased percent of cells with neutral invadopodia polarization compared to FN control surfaces; (ii) presence of a gradient of FN nanodots on a K-casein background increased percent of cells with negative invadopodia polarization compared to FN control surfaces; (iii) polarization of the golgi complex was similar to that of invadopodia in agreement with a spatial link; (iv) local adhesion did not necessarily appear to be a prerequisite for invadopodia formation.
URI: https://doi.org/10.1016/j.ejcb.2017.08.001
http://hdl.handle.net/11147/6843
ISSN: 0171-9335
1618-1298
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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