Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6069
Title: Genome-wide SNP discovery and genetic linkage map construction in sunflower (Helianthus annuus L.) using a genotyping by sequencing (GBS) approach
Authors: Çelik, İbrahim
Bodur, Sabahattin
Frary, Anne
Doğanlar, Sami
Keywords: Illumina Genome Analyzer II platform
Genetic linkage map
Genotyping
Next-generation sequencing
Single-nucleotide polymorphism
Helianthus annuus L.
Issue Date: Sep-2016
Publisher: Springer Verlag
Source: Çelik, İ, Bodur, S., Frary, A., and Doğanlar, S. (2016). Genome-wide SNP discovery and genetic linkage map construction in sunflower (Helianthus annuus L.) using a genotyping by sequencing (GBS) approach. Molecular Breeding, 36(9), doi:10.1007/s11032-016-0558-8
Abstract: Recently developed plant genomics approaches (LD mapping and genome-wide selection) require many molecular markers distributed throughout the plant genome. As a result, the availability of an increasing number of markers is essential for maintaining highly efficient and accurate plant breeding programs. In this study, we identified SNP loci in sunflower using a genotyping by sequencing (GBS) approach in an intraspecific F2 mapping population. A total of 271,445,770 reads were generated by the Genome Analyzer II next-generation sequencing platform and 29.2 % of the reads were aligned to unique locations in the genome. A total of 46,278 SNP loci were identified and 7646 SNP loci were validated in an F2 population. In addition, a SNP-based linkage map was constructed. This is the first report of SNP discovery in sunflower by GBS. The SNP markers and SNP-based linkage map will be valuable molecular genetics tools for sunflower breeding.
URI: http://doi.org/10.1007/s11032-016-0558-8
http://hdl.handle.net/11147/6069
ISSN: 1380-3743
1572-9788
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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