Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2125
Title: Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase
Authors: Koç, Ahmet
Mathews, Christopher K.
Wheeler, Linda J.
Gross, Michael K.
Merrill, Gary Frederic
Keywords: Cells
Enzyme kinetics
Enzymes
Genes
Mutagenesis
Synthesis (chemical)
Biochemistry
Issue Date: Jun-2006
Publisher: American Society for Biochemistry and Molecular Biology
Source: Koç, A., Mathews, C. K., Wheeler, L. J., Gross, M. K., and Merrill, G. F.(2006). Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase. Journal of Biological Chemistry, 281(22), 15058-15063. doi:10.1074/jbc.M601968200
Abstract: Thioredoxin was initially identified by its ability to serve as an electron donor for ribonucleotide reductase in vitro. Whether it serves a similar function in vivo is unclear. In Saccharomyces cerevisiae, it was previously shown that Δtrx1 Δtrx2 mutants lacking the two genes for cytosolic thioredoxin have a slower growth rate because of a longer S phase, but the basis for S phase elongation was not identified. The hypothesis that S phase protraction was due to inefficient dNTP synthesis was investigated by measuring dNTP levels in asynchronous and synchronized wild-type and Δtrx1 Δtrx2 yeast. In contrast to wild-type cells, Δtrx1 Δtrx2 cells were unable to accumulate or maintain high levels of dNTPs when α-factor- or cdc15-arrested cells were allowed to reenter the cell cycle. At 80 min after release, when the fraction of cells in S phase was maximal, the dNTP pools in Δtrx1 Δtrx2 cells were 60% that of wild-type cells. The data suggest that, in the absence of thioredoxin, cells cannot support the high rate of dNTP synthesis required for efficient DNA synthesis during S phase. The results constitute in vivo evidence for thioredoxin being a physiologically relevant electron donor for ribonucleotide reductase during DNA precursor synthesis.
URI: http://doi.org/10.1074/jbc.M601968200
http://hdl.handle.net/11147/2125
ISSN: 0021-9258
0021-9258
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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