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Title: Structure of Halothiobacillus neapolitanus Carboxysomes by Cryo-electron Tomography
Authors: Schmid, Michael F.
Paredes, Angel M.
Khant, Htet A.
Soyer Dönmez, Ferda
Aldrich, Henry C.
Chiu, Wah
Shively, Jessup M.
Keywords: Carboxysomes
Cryo-electron tomography
Electron cryo-microscopy
Issue Date: Dec-2006
Publisher: Academic Press Inc.
Source: Schmid, M.F., Paredes, A.M., Khant, H.A., Soyer Dönmez, F., Aldrich, H.C., Chiu, W., and Shively, J.M. (2006). Structure of Halothiobacillus neapolitanus Carboxysomes by Cryo-electron Tomography. Journal of Molecular Biology, 364(3), 526-53. doi:10.1016/j.jmb.2006.09.024
Abstract: Carboxysomes are polyhedral bodies consisting of a proteinaceous shell filled with ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). They are found in the cytoplasm of all cyanobacteria and some chemoautotrophic bacteria. Previous studies of Halothiobacillus neapolitanus and Nitrobacter agilis carboxysomes suggest that the structures are either icosahedral or dodecahedral. To determine the protein shell structure more definitively, purified H. neapolitanus carboxysomes were re-examined by cryo-electron tomography and scanning transmission electron microscopy (STEM). Due to the limited tilt angles in the electron microscope, the tomographic reconstructions are distorted. Corrections were made in the 3D orientation searching and averaging of the computationally extracted carboxysomes to minimize the missing data effects. It was found that H. neapolitanus carboxysomes vary widely in size and mass as shown by cryo-electron tomography and STEM mass measurements, respectively. We have aligned and averaged carboxysomes in several size classes from the 3D tomographic reconstruction by methods that are not model-biased. The averages reveal icosahedral symmetry of the shell, but not of the density inside it, for all the size classes
ISSN: 0022-2836
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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