Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2272
Title: Assessment of the molecular weight distribution of tannin fractions through MALDI-TOF MS analysis of protein-tannin complexes
Authors: Mané, C.
Sommerer, N.
Yalçın, Talat
Cheynier, V.
Cole, R. B.
Fulcrand, H.
Keywords: Aromatic compounds
Complexation
Fractionation
Molecular weight
Polymerization
Issue Date: Mar-2007
Publisher: American Chemical Society
Source: Mané, C., Sommerer, N., Yalçın, T., Cheynier, V., Cole, R. B., and Fulcrand, H. (2007). Assessment of the molecular weight distribution of tannin fractions through MALDI-TOF MS analysis of protein-tannin complexes. Analytical Chemistry, 79(6), 2239-2248. doi:10.1021/ac061685+
Abstract: An innovative mass spectrometry method was developed for determining mass distributions of tannin fractions that cannot be approached through direct MALDI-TOF analysis. It was applied to three procyanidin fractions with average degrees of polymerizations = 3, 9, and 28, respectively, and one gallotannin fraction (Tara tannin). The proposed approach consists of MALDI-TOF analysis of the soluble complexes formed between these tannin fractions and bovine serum albumin (BSA). Complexes were detected as an unresolved "hump" following the BSA signal, and spectra were mathematically processed to determine the parameters relative to the protein-tannin complexes, which are the number-average molecular weight (Mn), the weight-average molecular weight (Mw), and the polydispersity index (PI) for each tannin fraction. Regarding condensed tannins, results are consistent with those of the standard method (thiolysis followed by HPLC separation) for all tested fractions. The method was successfully applied to a hydrolyzable tannin fraction but no standard method is available for comparison.
URI: http://doi.org/10.1021/ac061685+
http://hdl.handle.net/11147/2272
ISSN: 0003-2700
0003-2700
1520-6882
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
2272.pdfMakale644.74 kBAdobe PDFThumbnail
View/Open
Show full item record



CORE Recommender

SCOPUSTM   
Citations

61
checked on Apr 14, 2023

WEB OF SCIENCETM
Citations

56
checked on Jan 19, 2024

Page view(s)

428
checked on Feb 19, 2024

Download(s)

418
checked on Feb 19, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.