Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4670
Title: Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from vibrio cholerae
Authors: Worthington, Erin N.
Kavaklı, İ. Halil
Berrocal-Tito, Gloria M.
Bondo, Bruce
Sancar, Aziz
Keywords: Photoreceptors
Dimers
Mutagenesis
Proteins
Negibacteria
RNA
Issue Date: Oct-2003
Publisher: American Society for Biochemistry and Molecular Biology
Source: Worthington, E. N., Kavaklı, İ. H., Berrocal-Tito, G. M., Bondo, B., and Sancar, A. (2003). Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from vibrio cholerae. Journal of Biological Chemistry, 278(40), 39143-39154. doi:10.1074/jbc.M305792200
Abstract: The sequence of Vibrio cholerae genome revealed three genes belonging to the photolyase/cryptochrome blue-light photoreceptor family. The proteins encoded by the three genes were purified and characterized. All three proteins contain folate and flavin cofactors and have absorption peaks in the range of 350-500 nm. Only one of the three, VcPhr, is a photolyase specific for cyclobutane pyrimidine dimers. The other two are cryptochromes and were designated VcCry1 and VcCry2, respectively. Mutation of phr abolishes photoreactivation of UV-induced killing, whereas mutations in cry1 and cry2 do not affect photorepair activity. VcCry1 exhibits some unique features. Of all cryptochromes characterized to date, it is the only one that contains stoichiometric amounts of both chromophores and retains its flavin cofactor in the two-electron reduced FADH2 form. In addition, VcCry1 exhibits RNA binding activity and copurifies with an RNA of 60-70 nucleotides in length.
URI: http://doi.org/10.1074/jbc.M305792200
http://hdl.handle.net/11147/4670
ISSN: 0021-9258
0021-9258
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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