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Title: Enalapril-induced apoptosis of acute promyelocytic leukaemia cells involves STAT5A
Authors: Purçlutepe, Özlem
İskender, Güniz
Kiper, Hatice Demet
Tezcanlı, Burçin
Selvi, Nur
Biray Avcı, Çığır
Kosova, Buket
Adan Gökbulut, Aysun
Şahin, Fahri
Baran, Yusuf
Saydam, Güray
Keywords: Acute promyelocytic leukaemia
Hl60 cells
Issue Date: Jul-2012
Publisher: International Institute of Anticancer Research
Source: Purçlutepe, Ö., İskender, G., Kiper, H. D., Tezcanlı, B., Selvi, N., Biray Avcı, Ç., Kosova, B., Adan Gökbulut, A., Şahin, F., Baran, Y. and Saydam, G. (2012). Enalapril-induced apoptosis of acute promyelocytic leukaemia cells involves STAT5A. Anticancer Research, 32(7), 2885-2894.
Abstract: Background: In this study, we aimed at evaluating the cytotoxic and apoptotic effects of enalapril on human HL60 acute promyelocytic leukaemia cells and at clarifying the roles of signal transducers and activator of transcription proteins (STATs) on enalapril-induced cell death. Materials and Methods: Cell viability and cytotoxicity tests were conducted by Trypan blue dye exclusion and 2,3-Bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5- carboxanilide inner salt (XTT) assays, respectively. Apoptotic analyses were performed by the AnnexinV-enhanced green fluorescent protein (EGFP) staining method and by fluorescence microscopy. Expression levels of STAT3, -5A and -5B genes were analysed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Results: The results showed that enalapril reduced viability and proliferation, and induced apoptosis in HL60 cells in a dose-and time-dependent manner as compared to untreated controls. The expression levels of STAT5A gene were significantly reduced in enalapril-treated HL60 cells as compared to untreated controls. Conclusion: Taken together, all data showed for the first time that enalapril has significant anticancer potential for the treatment of acute premyelocytic leukaemia.
ISSN: 0250-7005
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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