Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5603
Title: Step-by-step quantitative analysis of focal adhesions
Authors: Horzum, Utku
Özdil, Berrin
Pesen Okvur, Devrim
Keywords: Focal adhesion
Image processing
ImageJ
Particle analysis
Issue Date: 2014
Publisher: Elsevier Ltd.
Source: Horzum, U., Özdil, B., and Pesen Okvur, D. (2014). Step-by-step quantitative analysis of focal adhesions. MethodsX, 1(1), 56-59. doi:10.1016/j.mex.2014.06.004
Abstract: Focal adhesions (FAs) are specialized adhesive structures which serve as cellular communication units between cells and the surrounding extracellular matrix. FAs are involved in signal transduction and actin cytoskeleton organization. FAs mediate cell adhesion, which is a critical phenomenon in cancer research. Since cells can form many and micrometer scale FAs, their quantitative analysis demands well-optimized image analysis approaches [1-3]. Here, we have optimized the analysis of FAs of MDA-MB-231 breast cancer cells. The optimization is based on proper processing of immunofluorescence images of vinculin, which is one of the markers of FAs. All image processing steps are carried out using the ImageJ software, which is freely available and in the public domain. The advantages of our method are:The analysis steps are simplified by combining different plugins of the ImageJ program.FAs are better detected with minimal false negatives due to optimized processing of fluorescent images.This approach can be applied to quantify a variety of fluorescent images comprising focal and/or localized signals within a high background such as FAs, one of the many complex signaling structures in a cell.
URI: https://doi.org/10.1016/j.mex.2014.06.004
http://hdl.handle.net/11147/5603
ISSN: 2215-0161
2215-0161
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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