Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5644
Title: Gene reporter assay to validate microRNA targets in drosophila S2 cells
Authors: Akgül, Bünyamin
Göktaş, Çağdaş
Akgül, Bünyamin
Göktaş, Çağdaş
Keywords: Drosophila
Luciferase
Reporter assay
Target validation
MicroRNAs
Issue Date: 2014
Publisher: Humana Press
Source: Akgül, B., and Göktaş, Ç. (2014). Gene reporter assay to validate microRNA targets in drosophila S2 cells. Methods in Molecular Biology, 1107, 233-242. doi:10.1007/978-1-62703-748-8_14
Abstract: Bioinformatics programs have helped tremendously in identifying the targets of microRNAs, which are small noncoding RNAs that regulate gene expression posttranscriptionally. However, the partial complementarity between miRNAs and their targets hinders the accuracy of target prediction, necessitating the use of experimental validation procedures. Here, we describe a gene reporter assay typically used in our lab to validate putative miRNA-mRNA interactions in Drosophila S2 cells.
URI: http://hdl.handle.net/11147/5644
http://doi.org/10.1007/978-1-62703-748-8_14
ISSN: 1940-6029
1064-3745
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
5644.pdfMakale364.83 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

1
checked on Oct 16, 2021

WEB OF SCIENCETM
Citations

3
checked on Oct 16, 2021

Page view(s)

48
checked on Oct 22, 2021

Download(s)

34
checked on Oct 22, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.