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Title: Authentication of botanical origin in herbal teas by plastid noncoding DNA length polymorphisms
Authors: Uncu, Ali Tevfik
Uncu, Ayşe Özgür
Frary, Anne
Doğanlar, Sami
Keywords: DNA barcode
Capillary electrophoresis
Food genomics
Polymerase chain reaction
Issue Date: 1-Jul-2015
Publisher: American Chemical Society
Source: Uncu, A.T., Uncu, A.Ö., Frary, A., and Doğanlar, S. (2015). Authentication of botanical origin in herbal teas by plastid noncoding DNA length polymorphisms, Journal of Agricultural and Food Chemistry, 63(25), 5920-5929. doi:10.1021/acs.jafc.5b01255
Abstract: The aim of this study was to develop a DNA barcode assay to authenticate the botanical origin of herbal teas. To reach this aim, we tested the efficiency of a PCR-capillary electrophoresis (PCR-CE) approach on commercial herbal tea samples using two noncoding plastid barcodes, the trnL intron and the intergenic spacer between trnL and trnF. Barcode DNA length polymorphisms proved successful in authenticating the species origin of herbal teas. We verified the validity of our approach by sequencing species-specific barcode amplicons from herbal tea samples. Moreover, we displayed the utility of PCR-CE assays coupled with sequencing to identify the origin of undeclared plant material in herbal tea samples. The PCR-CE assays proposed in this work can be applied as routine tests for the verification of botanical origin in herbal teas and can be extended to authenticate all types of herbal foodstuffs.
ISSN: 0021-8561
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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