Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5987
Title: Cytotoxic and cytostatic side effects of chitosan nanoparticles as a non-viral gene carrier
Authors: Bor, Gizem
Mytych, Jennifer
Zebrowski, Jacek
Wnuk, Maciej
Şanlı Mohamed, Gülşah
Keywords: Cancer cells
Chitosan
Cytotoxicity
Gene delivery
Monocytes
Nanoparticles
Plasmid DNA
Issue Date: 20-Nov-2016
Publisher: Elsevier Ltd.
Source: Bor, G., Mytych, J., Zebrowski, J., Wnuk, M., and Şanlı Mohamed, G. (2016). Cytotoxic and cytostatic side effects of chitosan nanoparticles as a non-viral gene carrier. International Journal of Pharmaceutics, 513(1-2), 431-437. doi:10.1016/j.ijpharm.2016.09.058
Abstract: Although chitosan nanoparticles (CNs) became a promising tool for several biological and medical applications owing to their inherent biocompatibility and biodegradability features, studies regarding their effects on cytotoxic and cytostatic properties still remain insufficient. Therefore, in the present study, we decided to perform comprehensive analysis of the interactions between CNs–pKindling-Red-Mito (pDNA) and different cell line models derived from blood system and human solid tissues cancers. The resulting CNs-pDNA was investigated in terms of their cellular uptake, transfection efficiency, and physico-chemical, cytotoxic and cytostatic properties. The nanoparticles showed high encapsulation efficiency and physical stability for various formulations even after two days time period. Moreover, high gene expression levels were observed after 96 h of transfection. CNs-pDNA treatment, despite the absence of oxidative stress induction, caused cell cycle arrest in G0/G1 phase and as a consequence led to premature senescence which turned out to be both p21-dependent and p21-independent. Also, observed DNMT2 upregulation may suggest the activation of different pathways protecting from the results of CNs-mediated stress. In conclusion, treatment of different cell lines with CNs-pDNA showed that their biocompatibility was limited and the effects were cell type-dependent.
URI: http://doi.org/10.1016/j.ijpharm.2016.09.058
http://hdl.handle.net/11147/5987
ISSN: 0378-5173
1873-3476
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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