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Title: Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis
Authors: Yeşilırmak, Filiz
Doruk, Tuğrul
Yılmaz, Şerif
Tunca Gedik, Sedef
Keywords: Bacillus thuringiensis israelensis
Polyphosphate kinase
Secondary metabolite
Polyphosphate polymer
Issue Date: 2018
Publisher: TUBITAK
Source: Yeşilırmak, F., Doruk, T., Yılmaz, Ş., and Tunca Gedik, S. (2018). Comparative proteomic analysis of bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology, 42(1), 87-102. doi:10.3906/biy-1711-9
Abstract: Polyphosphate polymer (polyP) plays a very important role in every living cell. Synthesis of this linear polymer of phosphate (Pi) residues is catalyzed by the polyphosphate kinase (PPK) enzyme. It was shown that high levels of intracellular polyphosphate stimulated endotoxin production by Bacillus thuringiensis subsp. israelensis (Bti). In this study, proteomic analysis of the wild-type and two mutant strains, overexpressing the ppk gene (Bti pHTppk) and without the ppk gene (Bti ∆ppk), were used to clarify the relation between polyP and endotoxin production. Intracellular proteins were separated by two-dimensional gel electrophoresis; 41 spots of interest (proteins differentially expressed) were obtained and 35 of them were identified by mass spectrometry. Analysis of the protein profiles showed that there is a general decrease in the expression levels of proteins related with energy metabolism, amino acid metabolism, and purine biosynthesis in both Bti pHTppk and Bti ∆ppk. Gluconeogenesis and fatty acid metabolism were also slowed down in both strains, whereas expression of stress response proteins increased compared to the wild-type. These results suggested that changes in polyP concentration cause a general stress condition inside the cell, which in turn stimulates secondary metabolite synthesis
ISSN: 1300-0152
Appears in Collections:Chemistry / Kimya
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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